ATCC CRL-1992:
Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841
Salter RD, Cresswell P. Impaired assembly and transport of HLA-A and -B antigens in a mutant TxB cell hybrid. EMBO J 5(5):943-9, 1986. PubMed: 3522223
Steinle A, Schendel DJ. HLA class I alleles of LCL 721 and 174 x CEM.T2 (T2). Tissue Antigens 44(4):268-70, 1994. PubMed: 7871529
Weinzierl AO, et al. Features of TAP-independent MHC class I ligands revealed by quantitative mass spectrometry. Eur J Immunol 38(6): 1503-10, 2008. PubMed: 18446792
DSMZ no.: ACC 598:
established by PEG-mediated fusion of the B-lymphoblastoid cell line (B-LCL) LCL 721.174 with an 8-azaguanine and ouabain-resistant variant of the T-LCL CEM (CEMR.3); cells were described in the literature to synthesize HLA-A2 and -B5 RNA, but express only small amounts of A2 and no B5 antigen on the cell surface;
subclone of the T1 cell line which has lost both CEMR-derived copies of chromosome 6; cell line also known as 174xCEM.T2
The cell line is positive for EBV (HHV-4) by PCR analysis.
This line is a variant of the T1 cell line (ATCC CRL-1991) produced by selection the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR).
Together the T1 and T2 lines are useful for studying antigen processing and T cell recognition of class I MHC antigens.
