Details zum Vektor

Name	AAVS1-TRE3G-EGFP
Synonyme
Grundvektor
Bild
Funktion	Expression eukaryot
Herkunft	Addgene
Origin of Replication	pMB1/pBR322/pUC/ColE1
Referenz	Qian, K., Huang, C. T., Chen, H., Blackbourn, L. W., 4th, Chen, Y., Cao, J., Yao, L., Sauvey, C., Du, Z., & Zhang, S. C. (2014). A simple and efficient system for regulating gene expression in human pluripotent stem cells and derivatives. Stem cells (Dayton, Ohio), 32(5), 1230–1238.
Biologische Sicherheitsmaßnahme	✔
AZ ZKBS
Kurzbeschreibung	AAVS1-TRE3G-EGFP is a donor plasmid for the knock-in of an expression cassette into the adeno-associated virus integration site 1 (AAVS1). The expression cassette is flanked by two homological sequences to AAVS1. This cassette allows the expression of a puromycin resistance gene for selection, the Tet-on 3G tetracycline transactivator under control of the CAG promoter and EGFP under control of the tetracycline inducible TRE3G promoter. The vector backbone contains an ampicillin and kanamycin resistance gene and a pBR322 origin of replication.
Features	AmpR AmpR promoter pBR322 ori lac promoter T3 promoter Homology to AAV2 (left site) HA-L Splicing acceptor (SA) T2A-linker PuroR bGH polyA signal SV40 polyA signal small t intron EGFP TRE3G promoter with tet operator elements CMV enhancer chicken β-actin promoter chimeric intron Tet-on 3G (Tet transactivator TetR derivate) Β-globin polyA signal Lac promoter Homology to AAV2 (right site) (HA-R) T7 promoter ccdB ORF NeoR/KanR

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