Bundesamt für Verbraucherschutz und LebensmittelsicherheitZKBS

Vektorliste

Details zum Vektor

AdCMV5-GFP
  • adenoviral
  • viral
  • Qbiogene
First generation adenovirus vector. GFP recombinant adenovirus. The viral backbone is E1/E3 deletion, replication deficient, serotype 5 adenovirus. AdCMV5-GFP is a recombinant adenovirus in which E1 region has been replaced by a CMV driven GFP expression cassette. The viral backbone we use for our constructs is an Ad5 subtype delta L327, lacking a XbaI fragment of 1.89 kb deleting almost all of the E3 region from 28593-30471 (79 to 85 Map units). The backbone is also E1 deleted. The E1a and E1b deleted region is from 358-3332. These numbers are based on Ad5 wt sequence.

Ad5 ITR 1-103
Encapsidation signal 194-357
Globin Poly A 376- 477
GFP 1214-495
CMV5 promoter/enhancer 2280-1232
E2b 7541-3234
Late transcripts 5223-30084
E2a 26225-21548
E4 32901-30098
Ad5 ITR 33231-33129


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