ATCC® CRL-3250™
Cellosaurus: CVCL_CZ90
Gesek FA, et al. Mechanism of calcium transport stimulated by chlorothiazide in mouse distal convoluted tubule cells. J. Clin. Invest. 90(2): 429-438, 1992. PubMed: 1322939
Pizzonia JH, et al. Immunomagnetic separation, primary culture, and characterization of cortical thick ascending limb plus distal convoluted tubule cells from mouse kidney. In Vitro Cell Dev. Biol. 27A(5): 409-416, 1991. PubMed: 1649164
Rhim et al., Neoplastic Transformation of Human Epidermal Keratinocytes by AD12-SV40 and Kirsten Sarcoma Viruses., Science. 1985 Mar 8;227(4691):1250-2
PMID: 2579430
Schell et al., Potentiation of oncogenicity of adenovirus type 12 grown in African green monkey kidney cell cultures preinfected with SV40 virus. Persistence of both T antigens in the tumors and evidence for possible hybridization., Proc Natl Acad Sci U S A. 1966 Jan;55(1):81-8
PMID: 4287351
distale Tubuluszellen aus der Mausniere
immortalisiert mit Adenovirus 12 - SV40 Hybrid (Ad12-SV40)
209/MDCT is a continuous line of distal convoluted tubule (DCT) cells expressing a phenotype including NaCl cotransporter (Slc12a3) and PTH receptors. The DCT is a primary site for regulation of blood pressure by controlling NaCl absorption and for calcium homeostasis by parathyroid hormone. This cell line is of considerable use for studies of hormonal and pharmacological regulation of fundamental ion transport processes and their underlying mechanisms.
