Cellosaurus: CVCL_B5MU
Buc-Caron MH, Launay JM, Lamblin D, Kellermann O. Serotonin uptake, storage, and synthesis in an immortalized committed cell line derived from mouse teratocarcinoma. Proc Natl Acad Sci U S A. 87 (1990): 1922-1926.
PMID: 2155426
Murine neuronal cell line. A serotoninergic cell line, derived from a mouse teratocarcinoma. The clone 1C11 was immortalized through the expression of the SV40 T antigen.
The progenitor cell line F9K4b2 was obtained after transfection by pK4 of the embryonal carcinoma F9. pK4, a recombinant plasmid, carries the early region of SV40 under the control of the adenovirus 5 ElA promoter.This promoter is active in early embryonic cells. After in vitro differentiation of F9K4b2 was induced with retinoic acid and dibutyryl cyclic AMP (Bt2cAMP), several clones corresponding
to immortalized precursors of the neuroectodermal, mesodermal, and endodermal lineages were selected. One of the clones was cell line 1C11.
