Details zum Empfängerstamm
E. coli BT340 | |
F- λ- (φ80lacZΔM15) endA1 recA1 supE44 thi-1 gyrA96 hsdr17 relAl? ∆(lacZYA-argF)U169, pCP20 pCP20 carries the yeast Flp recombinase gene (FLP, aka exo), chloramphenicol and ampicillin resistant genes and temperature sensitive replicon pSC101 |
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K12 | |
1 | |
✔ | |
✔ | |
Synonym: DH5alpha/pCP20 E. coli K12-Derivat DH5α transformiert durch pCP20 (pSC101-Derivat mit Flp-Rekombinasegen aus Saccharomyces cerevisiae, λ cI857-Repressor, Temperatur-sensitivem Replikationsursprung und Ampicillin- sowie Chloramphenicolresistenzkassette); Az. 45110.1948 Referenz: Cherepanov PP, Wackernagel W. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene. 1995 May 26;158(1):9-14. |